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Removal of duplicate particles
===========================================
As we have picked particles by oversampling on the spheres, it is highly like that some particle positions will
converge to the same positions after the 3D refinement. To avoid unnecessary calculations and over-estimation of
final resolution, we need to remove them before trying to do a high resolution refinement. We do this by
calling the duplicate removal tools in RELION.

To do this in Doppio, go to :purple:`NEW JOB` tab in Doppio GUI and type **relion.select.removeduplicates** in the search bar.
In the first round, we've removed all the duplicated particles within 30 A radius. Our input parameters are given
in the image below:

.. figure:: ../_static/images/STA/select-dup30.-job-params.png
   :alt: Example image
   :width: 100%
   :align: center

     

After duplicate removal, our new particle file is ``Select/job013/particles.star`` and contains 8387 particles.

Also, refer `Relion duplicate removal documentation <https://relion.readthedocs.io/en/release-5.0/STA_tutorial/DuplicateParticles.html>`_.