15. Summary of the tutorial workflow
Below table summarizes the complete STA workflow of the tutorial data processing.
Operation |
Bin Factor |
Resolution (A) |
No. of particles |
|---|---|---|---|
Refine3D |
6 |
16.54 |
12623 |
Refine3D |
2 |
5.49 |
12623 |
Remove dup (30A) |
2 |
8387 |
|
Class3D & select |
2 |
4630 |
|
Refine3D |
1 |
5.18 |
4630 |
Remove dup (30A) |
1 |
4401 |
|
Postprocess (PP) |
1 |
5.08 |
4401 |
CtfRefine(cy1) + PP |
1 |
4.63 |
4401 |
Polish(cy1) + PP |
1 |
4.25 |
4401 |
Refine3D + PP |
1 |
4.25 |
4401 |
Original pixel size = 1.35 A (bin 1)
Complete workflow as generated by Doppio is show below
Doppio workflow