11. Removal of duplicate particles

As we have picked particles by oversampling on the spheres, it is highly like that some particle positions will converge to the same positions after the 3D refinement. To avoid unnecessary calculations and over-estimation of final resolution, we need to remove them before trying to do a high resolution refinement. We do this by calling the duplicate removal tools in RELION.

To do this in Doppio, go to NEW JOB tab in Doppio GUI and type relion.select.removeduplicates in the search bar. In the first round, we’ve removed all the duplicated particles within 30 A radius. Our input parameters are given in the image below:

Example image

After duplicate removal, our new particle file is Select/job013/particles.star and contains 8387 particles.

Also, refer Relion duplicate removal documentation.